Oral Presentation

¹Keyvan Khoshmaram; ¹Fatemeh Yazdian ; ²Hamid Rashedi

¹Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran

²Department of Chemical Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran

Controlled drug delivery systems are promising engineering technologies used in the treatment of many cancer tumors. This technology is for the targeted delivery and controlled release of therapeutic agents and has received great attention because of its advantages over chemotherapy. Breast cancer is the most common invasive cancer and the second leading cause of cancer death in women after lung cancer. This study aims to prepare a pH-sensitive nanocomposite of chitosan/agarose with exclusive properties in the field of cancer treatment as a carrier against breast cancer MCF-7 cells. 5-Fluorouracil, Curcumin, and a compound of both of them were loaded into the nano-carrier. Several analyses such as FTIR, DLS, and zeta potential were accomplished to confirm the efficiency of this carrier. In addition, the biocompatibility of nano-carrier and the effect of 5-Fluorouracil and Curcumin on cancer cells were evaluated with flow cytometry analysis which demonstrated that chitosan/agarose with Curcumin/5FU represents 58% less viable cells for the MCF-7 breast cell lines than chitosan/agarose loaded with 5-FU. Eventually, this study demonstrated that chitosan/agarose containing Curcumin/5-FU is an adequate candidate for the treatment of breast cancer cells.

Drug delivery system; Agarose; Chitosan; Breast cancer; Curcumin; 5-Fluorouracil; MC7-F