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Preparation of polyfam/MOF electrospun nanofiber as an efficient nanosorbent for micro solid phase extraction of three anticancer drugs followed by HPLC-UV analysis
Poster Presentation
Authors
1Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran
2a Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran
Abstract
" Cancer is a life-threatening disease and it has been one of the major causes of mortality among humans so far. Many efforts have been made to find a promising treatment for cancer by scientists. Application of anticancer drugs in chemotherapy is the main way for treatment of cancer. Due to high side effects, quantification of these drugs in human body and wastewaters is very important for controlling their level. The analysis of biological fluids such as serum, plasma and urine are commonly associated with the presence of various biologically active species, including proteins, salts, lipids, and the other compounds that can have a significant influence on the quantification of target analytes and can block columns and capillaries of the separation system. Therefore, sample pre-treatment step prior to analysis by analytical instrument is needed. Several sample preparation methods such as Liquid-Liquid Extraction (LLE), Solid Phase Extraction (SPE), micro-solid phase extraction have been developed for the extraction from different sample matrices. Several different sorbents have been used for extraction, and conductive polymers have been extensively taken into consideration to prepare nano-scale sorbents through the electrospinning method.
Electrospun Nanofiber polymers are great adsorbents for extraction of trace amounts of many different substances thanks to their high porosity and selective adsorption capability. Thus, they are used in various areas of research such as industry, environmental protection, medicine, and biotechnology. In this article a method is presented for the micro-solid phase extraction (µ-SPE) by using polyfam/ MOF electrospun nanofibers as an adsorbent for extraction of three anticancer drugs analytes. They were then quantified by High Performance Liquid Chromatography- Ultraviolet Detection (HPLC-UV). The sorbent was successfully synthesized by using a one-step method and it displayed high porosity, stability, hydrophilicity, and good capacity for extraction. Under optimal experimental conditions, the method has a wide linear range (b) satisfactory precision (with the intra-day and inter-day Relative Standard Deviations (RSD %) from about 5 and 9 respectively), (c) low limits of detection, (d) acceptable recoveries from spiked samples and (e) good regressions (R2=0.9996-0.9999). Eventually, the method was successfully used for the analysis of trace amounts of the above-mentioned analytes in pharmaceutical waste water, serum, plasma, and urine samples."
Electrospun Nanofiber polymers are great adsorbents for extraction of trace amounts of many different substances thanks to their high porosity and selective adsorption capability. Thus, they are used in various areas of research such as industry, environmental protection, medicine, and biotechnology. In this article a method is presented for the micro-solid phase extraction (µ-SPE) by using polyfam/ MOF electrospun nanofibers as an adsorbent for extraction of three anticancer drugs analytes. They were then quantified by High Performance Liquid Chromatography- Ultraviolet Detection (HPLC-UV). The sorbent was successfully synthesized by using a one-step method and it displayed high porosity, stability, hydrophilicity, and good capacity for extraction. Under optimal experimental conditions, the method has a wide linear range (b) satisfactory precision (with the intra-day and inter-day Relative Standard Deviations (RSD %) from about 5 and 9 respectively), (c) low limits of detection, (d) acceptable recoveries from spiked samples and (e) good regressions (R2=0.9996-0.9999). Eventually, the method was successfully used for the analysis of trace amounts of the above-mentioned analytes in pharmaceutical waste water, serum, plasma, and urine samples."
Keywords